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An electrophoresis of extracted DNA samples and PCR-fragments was performed in horizontal apparatus ("Helicon," Russia) in agarose gel with the use of 0.5Х ТBЕ buffer.
PCR products were electrophorized in 3% agarose gel in 0.5 × TBE buffer at 100 mV, in a horizontal apparatus (Horizon® 11 14; Life Technologies, Gibco BRL, Goettingen, Germany) and band intensities were measured using the Molecular Analyst Software (BioRad).
Equivalent amount of protein (10 μg) from each sample was electrophoretically resolved on 12.5% precast SDS-polyacrylamide gels (ExcelGel, GE Healthcare Biosciences) using horizontal apparatus (Pharmacia Biotech, Uppsala, Sweden).
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PCR products (4 to 12 uL) in individual samples, containing 1 to 3 uL blue juice 10× loading dye, were resolved on 2% agarose gels Horizon11-144 horizontal electrophoresis apparatus, Germany/TechnoSource mini horizontal electrophoresis apparatus, Mumbai, India), containing 0.5 ug/mL ethidium bromide with sterile 1XTAE (Tris-acetic acid-EDTA) running buffer, against 5 uL of markers (NEB).
A horizontal tube apparatus was used to test the effect of brown coal and two kinds of bituminous and anthracite on methane/air combustibility.
The amplified products (20.0 μl) were mixed with 4.0 μl of 6 X DNA loading dye (GeNei™) and were electrophoresed along with '100 bp Plus' DNA ladder (Thermo Scientific) in a horizontal gel apparatus (PowerPack™ Basic, Bio-Rad) using 2.5% agarose gel (containing ethidium bromide) in 1 X Tris-acetate-EDTA buffer pH 8.0 at 60 Volt for 120 min.
Kido et al. mechanically stimulated osteoblasts using a horizontal shaking apparatus fixed inside a tissue culture incubator to study how mechanical stimulation affects interleukin-11 expression [56].
Finally, the slides were washed in a TBE buffer (Tris base, Boric acid and EDTA) for 5 minutes and then the slides were submerged in TBE buffer in a horizontal electrophoresis apparatus.
Cells were then electrophoresed in Alkaline Electrophoresis Solution, pH>13 (300 mM NaOH, 1 mM EDTA) in a horizontal gel apparatus (Bio-Rad, Hercules, CA) at 1 V/cm for 30 minutes at 4°C.
We modified a homemade horizontal electrophoresis apparatus with platinum electrodes as an electrolyzer to perform cathodic reduction.
Gibco-BRL and Pharmacia make suitable small horizontal gel apparatus, and low-voltage (delivering 200 volts) power packs are suitable for these types of gel.
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