It has been noted that the arginine residues in the AT-hook sequence PRGRP insert their side chains into the minor groove of DNA and thus contribute directly to DNA binding, while lysines flanking the AT-hook core contribute to DNA binding by neutralizing negative charges of the DNA backbone [13], [17].
Interestingly, the AT-hook in the CbfA protein has the rather unusual core motif PKGRP, Residual activity of the R812A mutant of CbfA-CTD may be explained if we assume that at least lysine-810 within the AT-hook sequence KPK810GRPPKNLK of CbfA directly contributes to the specificity of DNA binding of CbfA-CTD in vivo.
An unrelated gene FliK regulates hook length; as its sequence is related to that of YscP, the similarly functioning molecular ruler for the injection tube of type III injectors [ 86], it is likely that precursors of the rod/hook proteins and a common system of length regulation were already present in the common ancestor of both – here argued to be a slime-secretion nozzle.
Participants in memory competitions that involve long sequences of arbitrary numbers are advised to "hook" the information on to easily recalled images.
HMGA2 binds strongly to the DNA through AT hook domains with KD values of 20 40 nM depending on the DNA sequence.
The CARMA subfamily GK or SH3-HOOK-GK sequences could not be superimposed on any annotated structure in de database used.
It was Franklin who invented the term "battery" when he hooked up Leyden jars in sequence and used the system to zap a chicken.
In order to examine possible seasonal changes in hook tool manufacture sequences, we re-plotted all sequences from Experiment 1 in temporal order, this time including incomplete behavioural actions and actions that were completed through multiple attempts, to highlight cases where birds may have struggled with the material (Fig. 5a).
In making the Adjuster fly in "Hero", both visual and practical effects were used; some of the simpler sequences involved Gauthier hooked up to a velocity rig, but in order to make him fly over the city skyline, a computer-generated avatar was created.
The first putative NLS (SSQK488KIKCHRCEKRFKKFS; NLS written in bold) is located outside of the CbfA-CTD as part of zinc finger region 2. The second putative NLS is located within the CbfA-CTD and covers the AT-hook within the sequence KK799RPIVLDNDKPKGR812PPKNLKEW (putative NLS in bold; AT-hook underlined).
Then Rita walks over to the window as if to look out, comes back to her radio, and through this one tense sequence, the audience is hooked.
