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To what extent the observed ∼2°C differences in the upper temperature limit for larval mouth hook movement behavior in rovers and sitters reflects fitness differences in nature remains to be determined.
We measured the persistence of rhythmic mouth hook movement.
Pauses in mouth hook movement occur occasionally although no difference in pausing was observed between rovers and sitters.
Failure of mouth hook movement was considered to have occurred when mouth hook movements stopped for at least 30 sec.
When we increased temperature linearly at 5°C/min (starting from 22°C) the natural rover variant, forR, exhibited mouth hook movement failure (see methods) at a significantly lower temperature than the natural sitter fors (∼2°C lower; Figure 1A).
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The frequency of mouth hook movements increased (>3 Hz) with increasing temperature until abrupt failure.
Mouth hook movements are easily visible in our preparation (see methods).
These results parallel those found for mouth hook movements (Figure 1A), our behavioral measure of thermotolerance.
The feeding rhythm of leafminer larvae was expressed as the frequency of oral hook movements per minute inside a leaf.
To determine if Drosophila for variants have different levels of thermotolerance, we developed a behavioral assay that allowed us to increase temperature systematically and record the temperature at which larval mouth hook movements failed.
Perhaps it is in their musicality; maybe it is an off-the-hook movement quality; sometimes it's even frailty.
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