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Near homogenous preparations of native complexes are obtained in sufficient amounts to perform biochemical assays and characterize their components.
The compact, multicellular in cell suspension spheroids are further purified by partitioning through various size (100 µm, 70 µm and 40 µm pore size; BD Biosciences) cell strainers to produce homogenous preparations of spheroid sizes of 40 µm–70 µm, 70 µm–100 µm and 100+ µm in diameter.
The method was a convenient way to produce homogenous preparations of spheres.
The protein is known to be a major obstacle in achieving homogenous preparations of plant-expressed biopharmaceuticals [ 37].
Moreover, in some lipase industrial applications this is not necessary because homogenous preparations are not required [ 19].
This resulted in near homogenous preparations of the two antigens, as evidenced by the SDS-PAGE profiles of the pooled peak fractions in Figure 2 (lanes marked 'P').
Similar(51)
To investigate the mechanism of degradation of high concentrations of formaldehyde by P. variotii NBRC 109023, we attempted to purify S-HMGSH dehydrogenase, a key enzyme of detoxification in eukaryotic organisms, and succeeded in obtaining an electrophoretically homogenous preparation of the enzyme.
Purification and labeling yielded a homogenous preparation containing a heterodimer of Cik1 and TMR-labeled Kar3.
Transgalactosylation reactions were performed on a 2-mL scale at 70 °C using 300 rpm agitation and 12 UoNPGal/mL final concentration of a homogenous preparation of HoBGLA.
Structural characterization of HCV envelope glycoproteins is challenging because of the difficulty in obtaining homogenous protein preparations [ 10, 21- 23].
Purification of independent homogenous GST preparations with differing substrate specificities indicated the presence of multiple forms of GSTs [ 3].
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