In contrast to previous reports, this method does not require designing dye-labeled primers, and gel electrophoresis and isolation step after PCR were avoided in this homogenous method.
As a particular case, if c1 = c2 and m = n, the explicit solitary wave solution of GKP goes to the one obtained by Dai et al. [30] u = pm left( {frac{{(m + 1)(m + 2)hsec h^{2} left( {frac{m}{2}zeta sqrt {frac{h}{{beta p^{4} }}} } right)}}{{2alpha p^{2} }}} right)^{frac{1}{m}}, (15)using auxiliary and homogenous method.
High-density lipoprotein (HDL) cholesterol was measured using a homogenous method with polyethylene glycol modified enzymes and sulphated α-cyclodextrin.
The relative affinities of these biotin derivatives were consistent with those estimated by a homogenous method using their purified counterparts as samples (Table 3)[ 32].
Relative affinities of candidate biotin derivatives with unknown molar quantities in each mixture sample were consistent with those estimated by a homogenous method using their purified counterparts as samples.
(c) The average, or any one, of the stable relative affinities of each candidate biotin derivative (BCHA, BDETA, or BME) on the response curve was taken as the index of its relative affinity, and this relative affinity was consistent with that obtained using a homogenous method and its purified counterpart as the sample (Table 2)[ 32].
Homogenous fluorescence methods are providing an important tool for HTS technologies.
In addition, the modulation of TPM dye/G-quadruplex complex fluorescence by Ag+ and Cys could be used to develop a simple, fast, label-free and highly specific homogenous sensing methods for Ag+ and Cys.
Homogenous detection methods such as TaqMan® [ 20] and molecular beacon -based [ 21] approaches provide uniplex reactions, and are readily applied to a large number of samples but multiplexing may be difficult.
Total cholesterol, high density lipoprotein (HDL) and triglycerides were assayed on fresh fasting serum using CHOD-PAP, a homogenous direct method (Abbott Diagnostics, Berkshire, UK) and GPO-PAP methods respectively in Norfolk.
