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Using simulation, design of experiments and response surface methodology, various homogenous lines controlled by Kanban, CONWIP and the hybrid Kanban/CONWIP control mechanisms are optimized and then compared.
In the course of this procedure, cloning of primary heterogeneous suspensions generated secondary homogenous lines.
Of these, we selected the two more homogenous lines per subject which were thoroughly characterized and shown to be fully reprogrammed to pluripotency.
Although our cloning procedure did not, against expectations, lead exclusively to lines with homogenous GFP expression, clearly the cloning method is an effective way to substantially increase the number of homogenous lines.
Because coding sequences are under constraint, they tend to be less variable and, at least in this instance, seem to be reasonably well resolved without the time-consuming measures needed to create genetically homogenous lines.
Because the cloning procedure can significantly increase the frequency/yield of homogenous lines, it is of high general impact for both molecular and biochemical analyses of BY-2 transgenic lines.
Similar(54)
Furthermore, the instrument must have a homogenous line integral over the beam cross-section.
In order to obtain a more homogenous line, calli from suspension cultures were reselected and four promising cell lines were obtained, but only the best-performing line was subcultured in liquid medium.
As variance is not homogenous across lines (some lines have near zero variance), the proportional data are very non-normal, and sample sizes differ across lines, we determined the statistical significance of the among-genotype variance by resampling data 100,000 times and partitioning variance among this permuted data.
Appearance of tiller-spreading phenotypes in 6 homogenous mutation lines further supported the conclusion.
An alternative explanation may be that contamination by normal (diploid) cells in primary tumors might decrease the sensitivity of detection of homozygous losses compared to homogenous cell lines.
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