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After 24 hours cells were harvested and resuspended in 5× cell volumes of hypotonic buffer (10 mM HEPES pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, complete 1× protease inhibitor cocktail) allowed to swell on ice for 10 min. Cells were transferred to a glass Dounce homogenizer and homogenized through 25 up-and-down strokes using the tight pestle.
Finally, the dispersion was homogenized through an ultrasound cell cracker (SCIENTZ IID, Scientz Biotechnology, Ningbo, China) to obtain insulin-loaded BLPs.
If we continue to allow the taste of our food to be homogenized through the use of added sugar, we will not only rob ourselves and future generations of a vast and diverse culinary tradition, but an integral part of our human experience.
Cells were then homogenized through a cell strainer, and the resulting suspension was spun down.
Spleens, tumor-draining inguinal lymph nodes, and tumors were homogenized through 0.22 µm strainers to produce single cell suspensions.
Extracts were homogenized through a narrow gauge syringe, incubated for 10 min on ice and clarified by centrifugation at 13,000×g for 10 min at 4°C.
Similar(27)
As Markov transition probabilities homogenize through diffusion, an implicit clustering emerges from the network.
After homogenizing through sterile blender, the mycelia were washed with sterile distilled water three times under laminar flow cabinet.
Half an amount of each sample was placed directly into a sterile bag and sent to the laboratory, homogenized, sieved through a 2-mm filter, and stored in an icebox at 4 °C for microbial analysis.
The sample was homogenized, run through a syringe, and centrifuged at 6,000 x g for 15 mins.
The cell suspension was homogenized, filtered through 40- μm cell strainers (BD Biosciences, Le Pont de Claix, France), and plated in growth culture medium.
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