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For muscle RNA extraction, 30 mg of tissue (Patients 1 4, and three control subjects) was homogenized over ice using a Potter-type tissue homogenizer in RLT buffer (from RNeasy® Mini Kit, Qiagen) with 0.01% 2-mercaptoethanol.
Muscle tissue from Patients 1, 2, 4, 5 and 7 and three control subjects (30 mg) was homogenized over ice using a Potter-type tissue homogenizer in buffer containing 250 mM sucrose, 50 mM Tris-HCl pH 7.4, 5 mM MgCl2 (all Sigma) and protease inhibitor cocktail tablets EDTA-free (Roche).
We found a high degree of variability in ROS positive cells within negatively isolated neutrophils, which was further increased and homogenized over almost the entire population by the addition of either PMA or fMLP, while LPS did not increase the level of ROS (Fig. 3C).
For purification of His6-Bgl1, the cell pellet was washed, suspended in 50 mL phosphate buffer (50 mM, pH 7.4) and homogenized over a 3-min period using a MP FastPrep-24 Instrument.
Previous studies using transplanted individuals have indicated that pools located on the same rocky outcrop will tend to become genetically homogenized over periods as short as months [ 9]. Between two and ten other sites from disjunct outcrops were sampled from the vicinity of each of the four focal sites within each region.
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We formulate a level set description to capture multi-valued solutions to the band WKB system, and then evaluate total homogenized density over a sample set of bands.
Every day brings news of old favorites closed, familiar neighborhoods homogenized, ethnic enclaves turned over to the legions of Capital, not to mention Oberlin and Bard.
But rather than a monotonously uniform surface homogenized by impact cratering over the eons, the first up-close look at the asteroid reveals a full palette of mineral "colors" (mapped here in false color reflecting the wide range of rock compositions).
The pellet was homogenized in TL20, laid over a 36% sucrose cushion, and recentrifuged.
But I also want to reiterate my original point, which is simply that I think it is possible to over-homogenize an idea of how academics identify, or for that matter, how people in general identify in political terms.
It's important to note here the alluring temptation to over-homogenize Africa.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com