Sentence examples for homogenized manually from inspiring English sources

A known weight of placental biopsy was homogenized manually in four volumes (w/v) of Tris buffered saline containing EDTA-free serine-/cysteine-protease inhibitor cocktail, diluted according to the manufacturer's instructions (Roche Biochemicals, Indiana, USA) using a homogenizer.

After two time dilution, the homogenate was then centrifuged at 2000×g for 9 min. The supernatant was decanted and centrifuged at 12000×g for 11 min. To permeabilize synaptosomes, the pellet was suspended in isolation buffer supplemented with digitonin (0.2 mg/ml) and homogenized manually.

Tissue was homogenized manually in liquid nitrogen before extraction.

A bale was homogenized manually, aliquoted in plastic bags, and stored at ambient temperature.

Tissue (200 mg) samples were homogenized manually in 2 mL of homogenizing buffer (100 mM KH2PO4 K2HPO4, pH 7.4, plus 0.1% [w/v] digitonin).

Tumor tissue was homogenized manually using mini-pestils and RNA extracted using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.

Xenograft tumor tissue was dissected, manually homogenized (glass homogenizer) and incubated for 30 min in protein lysis buffer (20mM Hepes (pH 7.5), 2mM EGTA, 10% glycerol, 1% Triton×100, 1mM PMSF, 200µM Na3VO4 [all Sigma], and protease inhibitors [Complete Protease Inhibitor Cocktail, Roche]) on ice.

Frozen tumors were manually homogenized with homogenization hammer and tissue powders were resuspended in 500 μl of PBS.

Tissue (1 × 2 mm) or cell line lysates were manually homogenized (PYREX homogenizer; VWR, Radnor, PA, USA) and prepared as previously described [ 32].

The kidneys of 12 16 wk old db/+ and db/db mice were collected, and cleaned slices (10 25 mg) of kidney tissues were homogenized in 1 ml RIPA buffer with a glass-glass homogenizer manually for 30 strokes.

Prior to sampling, the soil was sieved to 2 mm (steel mesh) and manually homogenized.