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Briefly, total pulmonary artery tissues were frozen in liquid nitrogen and then homogenized into homogenization buffer containing 50 mM Tris-HCl, 150 mM NaCl, 100 mM NaF, 1% Triton X-100, 1 mM dithiothreitol, 0.1 mM phenyl methylsulfonyl fluoride, 1 mM leupeptin, 0.02 M Hepes, 10−3 M EDTA, 10−3 M EGTA, and 20% glycerol, followed by centrifugation to 10000 ×g by 10 min.
Samples were prepared from lyophilized leaf tissue essentially as described [ 34], except that dried tissue was homogenized into a powder in a FastPrep homogenizer before adding buffer, and 20 mg of this powder was used to prepare DNA.
The brain tissue was homogenized into three fractions as described previously [44], [45] to provide homogenates that were soluble in tris-buffered saline (TBS), TBS containing 1% (w/v) Triton X-100 (TX) and an SDS-solublized pellet fraction (SDS).
The biopsy piece was homogenized into phosphate buffer saline (PBS) in an all glass disposable homogenizer.
It would be a sad world were the races homogenized into one amorphous mass.
America has never been a melting pot, where people of diverse cultures are homogenized into a undifferentiated mass.
Similar(11)
The contents were weighed (W2) followed by homogenizing into a paste.
Hold it here for another 30 seconds to homogenize into a smooth, creamy smoothie.
The big mass-market brewers had homogenized beer into a tepid pale lager.
Normal conjunctiva and pterygium tissue were homogenized individually into RIPA lysis buffer (Santa Cruz Biotechnology).
Cells were homogenized directly into the following buffer: Tris 50 mM, NaCl 150 mM, EDTA 10 mM, and Triton-X 1%, and centrifuged at 10 000 × g for 2 min.
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