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Prior to dissolution, the soil samples were homogenized according to the grab-sampling procedure developed by Pierre Gy [12].
For native co-immunoprecipitation experiments whole mouse brains were homogenized according to the 'NativePAGE sample preparation kit' (Invitrogen) instructions.
Myeloperoxidase (MPO) was measured in tissue samples (healthy vs the final IR jejunum sample) that were homogenized according to the later described Western blot protocol.
The frozen brain halves of the intranasally treated animals were homogenized according to the protocol published by Shankar et al [46].
The tissue samples were homogenized according to Machado et al. [ 29].
They were then homogenized according to the method [ 17] for the preparation of 40% (w/v) liver homogenates in methanol.
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Citrate synthase activity was determined in homogenized tissue according to the method of Srere [ 24].
Total RNA was extracted from homogenized tissue according to the protocol provided in the RNeasy tissue Mini Kit (Qiagen, Hilden, Germany), reverse transcribed using 500 ng RNA as template and oligo-dT and Random Hexamers (Ration 1 2) as primers to obtain cDNA fragments.
The rotifers were homogenized with 0.8 mm glass beads using a vortex mixer and total RNA was isolated from the homogenate according to the manufacturer's instructions.
Lung tissue and BAL samples were homogenized and decontaminated according to a previously reported procedure [61], whereas lymph node samples were not.
Total RNA was extracted from cells with TRIzol reagent (Invitrogen, Carlsbad, CA) or from tissues homogenized in TRIzol according to the manufacturer's instructions.
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