We also study the stability under homogenization of this region, meaning that whenever one homogenizes a mixture of two materials belonging to it, the effective diffusion tensor also belongs to this region.
Three root samples were ground in N2 and homogenized in a mixture containing 1 mL NTES buffer and 0.7 mL of phenol: chloroform (1 1).
Brieflly, BAT interscapular and liver were removed and homogenized in a mixture containing 0.32 mM sucrose, 1 mM EDTA, 10 mM MOPS/Tris buffer pH 7.4, and 0.2 mg/ml of non-delipidated BSA (Fraction V-Sigma A7906-50G).
To remove polysaccharides and polyphenolics the tissue was homogenized in a mixture of RNA Isolation Aid (Ambion®, USA) and RNAqueous® Lysis/Binding solution.
Briefly, about 10 50 mg of whole seeds or seed parts from each stage were ground into powder in liquid nitrogen and then homogenized in a mixture of the lysis solution from the mirVana miRNA isolation kit and Plant RNA Isolation Aid.
Adult male Wistar rats were killed by decapitation, and BAT was immediately removed from interscapular deposits and homogenized in a mixture containing 0.32 mM sucrose, 1 mM EDTA, 10 mM Mops/Tris buffer (pH 7.4) and 0.2 mg/ml of non-delipidated BSA (Fraction V-Sigma A7906-50G).
The dry material was homogenized by sonication in a mixture of carbon tetrachloride and heptane, and fractionated by density gradient centrifugation.
Initially, 125 mg of samples was homogenized (Ultra-turrax, IKA T10 Basic) with a mixture containing chloroform (125 μL) and methanol (200 μL).
The brain was removed and homogenized in cold PBS, containing a mixture of protease inhibitors (leupeptin, pepstatin, and aprotinin) prior to centrifugation at 15,000 ×g for 15 min. The resultant supernatant was collected for further analysis.
Cleaned vessels from the four experimental groups (n = 4 per group) were homogenized in RIPA solution containing a mixture of protease inhibitors at low speed (between 10 000 y 15 000 rpm during 15 seconds for each pulse) followed by 10000 rpm for 10 min at 4°C centrifugation.
Briefly, the culture was mixed with one volume of ethyl acetate and strongly shaken by hand in an extraction funnel to homogenize the mixture.
