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After homogenization, 1 mL of CHCl3 and CCl4 each were added per gram of sample, then mixed.
Following homogenization, 1 ml of solution was transferred to a 1.5 ml Eppendorf tube and centrifuged at 12,000 g for 10 minutes at 4°C to remove insoluble material.
After homogenization 1 mg was taken and used for each extraction.
After homogenization, 1 ml of sperm solution was filtered through a nucleopore membrane (0.2 μm) and fixed in 2.5% glutaraldehyde, 2% paraformaldehyde and PB 0.1 M solution.
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In brief, cells were incubated with Reagent A for 2 min on ice and then transferred to Dounce homogenizer for homogenization (20 strokes).
[16] At the time sequence homogenization occurred (t=0), n1 was 1, and n2, n3, and n4 were zero.
Following homogenization, 300 μL of homogenate was processed according to manufacturer's instructions, using the Spin Procedure.
Following homogenization 750 µl of the homogenate were transferred into a 1.5 mL Eppendorf and 250 µl TCA were added, subsequently the mixture was vortexed thoroughly and centrifuged 10 minutes at 4,000 g.
Following the tissue homogenization, 0.2 ml chloroform was added to homogenate, shaken vigorously, incubated at room temperature for 2-3 minutes and microfuged at 12,000 g for 15 sec.
As exemplified in Figure 3, the fraction of each component depends on (1) the treatment applied to the fibres before homogenization, (2) the number of passes through the homogenizer and (3) the pressure applied during homogenization.
High pressure homogenization [8] and wet milling [9] are examples of such methods applied to curcumin.
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