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More than 95% of the parasites were strongly and homogeneously stained by CFSE, and non-stained parasites were easily distinguished (Fig. 1A, 1B).
However, the Ccml of Sf appeared perturbed displaying reduced density as evidenced by the light and sparsely stained regions (arrowheads in Figure 2f), in contrast to the relatively dark and homogeneously stained Ccml in the native cell walls.
A few homogeneously stained glial cells could also be found.
Germinating conidia, while phagocytized by neutrophils, showed compartmentalized neutral red whereas homogeneously stained conidia remained ungerminated (Fig. 1E inset).
The striosomes in the CN body are homogeneously stained for ENK and contain none or very few ChAT-ir neurons (Figs. 4C, 5B).
When nuclei were completely and homogeneously stained and when multiple small spots or single large signals were present, the sperm cells were classified as positive.
Similar(32)
Amplified sequences may be found within the chromosomes, clustered within homogeneously staining regions (HSRs) or spread among different loci.
Chromosomes with homogeneously staining region (HSR) were identified with the analysis of FISH metaphases in inverted digital images.
Recently, Del Rey et al. found that CCND1 amplification in homogeneously staining regions was present in 6 out of 14 CIN-positive tumors; 3 of them also showed amplification of this gene in double minutes [35].
This is accentuated somewhat in mouse PIN, since in normal mouse nuclei there are highly characteristic darkly staining punctate areas of chromatin that are distributed throughout the more homogeneously staining regions, and, these punctuate areas are largely absent in the mouse PIN cells (Figures 1 2).
In LAU-Me275, the fluorescence signal indicated that at least 8 MDM2 copies were present at the locus on Chr12 in addition to a homogeneously staining region on Chr5 (see Fig. 3A and 3B) which is in agreement with results from the SNP arrays.
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