Sentence examples for homogeneously linked from inspiring English sources
To test the feasibility of top-down UVPD-MS for determining linkage stoichiometry, homogeneously linked Lys48 and Lys63 ubiquitin tetramers were purified and mixed in known molar ratios.
The method requires that the two oligomers have similar ionization efficiencies, and analysis of the homogeneously linked tetraubiquitin chains showed that this is indeed the case (see Figure S2 in the Supporting Information).
This method capitalizes on both the critically important intact mass measurement of top-down MS to describe chain length and the almost-complete backbone fragmentation provided by N-terminally derived ions after UVPD to detect the ubiquitin linkage pattern, especially for homogeneously linked chains.
The pattern becomes even more pronounced for longer homogeneously linked ubiquitin chains (see Figures 2C F), and the change in ion current from regions in the protein that are specifically indicative of the sites of conjugation can be used in a semiquantitative manner (see discussion below).
Using these data in conjunction with the intact mass of the precursor and high observed ion current up to and including the a62 ion, there was ample evidence to confirm that this substrate was homogeneously linked via Lys63 linkages and contains three ubiquitins.
Using VMS VAX technology, the acquisition system (CAMAC) and control system (BITBUS) were connected to controllers situated in the machine bus of these machines that were linked homogeneously by the VMS 'cluster' technology.
To construct these reagents, homogeneously modified mononucleosomes were linked using T4 DNA ligase.
However, the core set of pathways under strong microRNA control appear to be homogeneously enriched throughout the majority of diseases, since many diseases are linked to a large number of microRNAs.
The percentage of positive cells for Maspin or Bax, respectively, was linked with the intensity of staining classified into four groups: 0 -negative; 1 -slight positive (light brown color of the either homogeneously or spotty stained cells); 2 -distinct positivity (medium brown homogeneously stained cells); 3 -strong positivity (dark brown to black homogeneously stained cells).
Non-hub nodes are divided into four classes: (R1) nodes with all their links within their module (P ≤ 0.05); (R2) nodes with some links to other modules (0.05 < P ≤ 0.62); (R3) nodes with many links to other modules (0.62 < P ≤ 0.80); (R4) nodes with links homogeneously distributed among all modules (P > 0.80).
