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TEM samples were prepared using ultrathin carbon on holy carbon copper grids.
The samples for TEM were prepared by drop casting Sn NCs/ethanol colloids on holy carbon mesh/Cu TEM grids and then were allowed to dry.
3xflag-LRRK2 purifiedpurified with optimized lysis, wash and elution buffers (see above) was applied to glow-discharged thin (5 nm) carbon films supported by holy carbon Quantifoil grids, vitrified and imaged as described in Materials and Methods.
For cryo-grid preparation, the Quantifoil 1.2/1.3 holy carbon grids were baked at 50°C in an oven for 2 weeks.
For soluble coatomer, holy carbon grids coated with thin (5 nm) continuous carbon films, which was manufactured in-house, were used.
Substantial discrepancy between measured thickness of holy carbon grids from this study and that of manufacturer's description is possibly due to residual plastic layer underneath carbon film (Ermantraut et al. 1998), which may have hampered correct EELS measurement.
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Figure 8h gives a typical low-magnification TEM image of a triangular WS2 flake on a holy carbon-coated copper grid.
CuS nanoparticles were further investigated using electron energy loss spectroscopy (EELS) by isolating a nanoparticle in a holy-carbon TEM grid to identify the compound correctly.
This is the exclusive, short story of how Diageo North America, with creativity and guts, both in operations and in the senior ranks, achieved the holy grail of carbon emissions reductions.
Obtaining reliable estimates of AGB using EO data can be considered the holy grail of forest carbon science.
The Vatican was told that an entire year's worth of its emissions would soon be secured in those trees -- making the Holy See the first carbon-neutral sovereign state.
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