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The compressive phase was subdivided herein into the shooting, grasping, and holding stages.
The design consists of an Inverted Pendulum (IP) holding stages of Geometrical Anti-Spring Filters (GASF) and pendula, which isolate the test mass suspension from ground noise.
A novel particle-based discrete element model (DEM) is developed to simulate the whole Direct Metal Laser Sintering (DMLS) process, which includes simplified powder deposition, recoating, laser heating, and holding stages.
However, as different events were recognized in the compressive and recovery phases of the goblin shark, the compressive phase was herein subdivided into the shooting, grasping, and holding stages, and the recovery phase was subdivided into the re-opening and re-closing stages.
The RQ-PCR consists of two holding stages of 1 cycle each at 50 °C for 2 min followed by 95 °C for 10 min and a cycling stage at 95 °C for 15 s and 60 °C for 1 min (50 cycles) in a StepOnePlus Real-Time PCR system (Applied Biosystems, Darmstadt, Germany).
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The gape angle (Fig. 2c) was maintained at 6 10° throughout the holding stage.
During the holding stage, creep compliance increases slowly at first and then increases quickly with modulus loss.
These changes observed in holding stage decreases with decrease of stretching speed and temperature and with increase of total strain.
At lower strains the films were found to continue to change significantly during holding stage leading to increase in birefringence.
The holding stage is the last stage of the compressive phase, defined as the duration that the jaws are kept closed after the grasping stage.
The real-time PCR amplification included a holding stage at 95°C for 10 min, followed by the cycling stage with 40 cycles of 95°C for 15 sec and subsequently 60°C for 1 min.
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