This tripartite ring-shaped molecule is involved in holding replicated sister chromatids together from S phase until anaphase onset.
In PAUP, the number of ILD replicates was set to 1,000, with 10 random addition sequence replicates, holding one tree per replicate.
Branch support was estimated through 1 000 jackknife replicates with the JAC resample method, independently deleting 37% of the characters in each step [ 54]; the heuristic search settings for the jackknife analysis were set to 100 random addition replicates holding one tree per replicate and TBR branch swapping with at most five trees saved per replicate.
MP analyses were performed using PAUP* 4.0b10 [37], with the following parameters for the heuristic search: 1000 random addition replicates holding 100 trees per replicate, tree-bisection-reconnection (TBR) branch swapping and the options Multrees and Steepest Descent in effect.
MP tree searches were conducted using 1,000 random addition tree-bisection-reconnection (TBR) searches in PAUP* v4.0b10 [ 92] with a maximum of ten trees held per replicate.
An initial heuristic search was carried out with 1000 replicates, holding a maximum of 10 trees per replicate.
Each of the 11 plates used in this pilot study held 4 replicates of 3 crystallisation conditions for 6 proteins.
The cohesin ring holds newly replicated sister chromatids together until their separation at anaphase.
They are thought to hold together replicated sister chromatids by entrapping both sister DNAs within the ring-shaped architecture created by their Structural Maintenance of Chromosomes (called SMC1 and SMC3) and kleisin (called RAD21 or SCC1) subunits (reviewed in [ 88, 126, 127]).
The relative robustness for clades found in all single most parsimonious trees (MPTs) was assessed by performing 1000 replicates of bootstrapping using fast, stepwise additions, TBR branch-swapping with 10 random taxon additions per replicate, MULTREES on and holding 100 trees per replicate.
