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After amplification, a fusion curve was obtained according to the following protocol: 10 sec holding followed by a 0.05°C increase, repeated 80 times, and starting at 55°C.
After amplification, a fusion curve was obtained using the following protocol: 10 sec holding followed by a 0.5°C increase, repeated 80 times and starting at 55°C.
After amplification, a fusion curve was obtained in order to ensure that a single PCR product had been generated using the following protocol: 10 sec holding followed by a 0.5°C increase, repeated 80 times and starting at 55°C.
He sold $225 million worth of shares in search-engine company Naver in October 2014, but Naver is still his biggest holding, followed by NHN.
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A consultation was subsequently held, followed by a Treasury report.
In 1878 the Scottish Championships were held, followed in 1879 by the Irish Championships.
If there are other qualified bidders, an auction would be held, followed by a court hearing to approve the sale to the winning bidder or bidders.
I stood and watched visitors' reactions for a while: a first glimpse would stop them in their tracks, and then confusion would take hold, followed by intense discussion.
Birling rules became standardized, and camp championship contests were held, followed by intercamp and eventually intersectional contests, finally culminating in national championship tournaments, called rodeos.
That gun fired 31 bullets the morning of Nov. 25, 2006, first the 16 bullets that the gun holds followed by the 15 bullets in the new magazine that Detective Oliver loaded and emptied.
Mr. Gersten, who was an associate producer at the Public (the title his daughter now holds) followed his tenure there working with the producer Alexander Cohen and as executive vice president of Francis Ford Coppola's Zoetrope Studios.
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