Sentence examples for hold steps from inspiring English sources
The phrase "hold steps" is not correct and does not convey a clear meaning in written English.
It may be intended to refer to maintaining or following a series of steps in a process, but it lacks clarity and proper usage. Example: "Please hold steps until we receive further instructions."
The integration of up- and downstream unit operations can result in the elimination of hold steps, thus decreasing the footprint, and ultimately can create robust closed system operations.
A slow curing cycle with hold steps at 80, 110, 140 and 2000 °C was used for the sample being pressed isostatically in a steel mold.
These data indicate that refrigerated hold steps could be incorporated into the manufacturing process without any detrimental impact on the integrity of the protein.
The amplification conditions were set as followings: initial hold steps (50°C for 2 min, then 95°C for 10 min) and 40 cycles of a two-step PCR (95°C for 15 s and 60°C for 1 min).
The amplification reactions were performed in a MJ Research DNA Engine thermocycler (Bio-Rad, Hercules, CA, USA) with two initial hold steps (50°C for 2 minutes, followed by 95°C for 10 minutes) and 40 cycles of a two-step PCR (92°C for 15 seconds, 60°C for 1 minute).
The amplification reactions were carried out in an ABI Prism 7700 Sequence Detection System (Applied Biosystems) with two initial hold steps (50°C for 2 min, followed by 95°C for 10 min) and 50 cycles of a two-step PCR (95°C for 15 s, 60°C for 1 min).
These enzyme mixtures were then supplemented with twice desalted Cellic® CTec2 from Novozymes (Bagsvaerd, Denmark) after the initial 24-h high temperature hold step.
For thermal denaturation, the samples were heated from 20°C to 75°C with stepwise increment of 0.5°C per minute and a 30 s hold step for every point, followed by the fluorescence reading.
We initially used a high temperature enzyme loading of about 15% of the total enzyme loading to test the efficacy of the high temperature hold step at a low solids loading.
Employing a higher operating temperature hold step will enable thermophilic enzymes to be added earlier in the process, resulting in both time savings and improved conversion efficiency compared to using current mesophilic, commercial enzyme cocktails.
The samples were heated from 20°C to 75°C with stepwise increment of 0.5°C per minute and a 30 s hold step for every point, followed by the fluorescence reading.
