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Results showed that localization of PLP in GFP-MAL2/HOG cells was similar to that observed in HOG cells.
HOG cells were described previously [21], and 293T cells were obtained from ATCC (Manassas, VA).
Similar extracellular vesicles containing PLP have also been observed in HOG cells.
The HOG cells were kindly provided by Anne Lise Hestvik and Dr Campagnoni.
GFP-MAL2/HOG cells consisted of HOG cells stably transfected with a chimera consisting of green fluorescent protein (GFP) fused to the amino-terminal end of MAL2 (GFP-MAL2) [26], [26].
HOG cells have been also proved to form myelin-like membrane sheets which, in addition, accumulate myelin proteins such as MAL [26] and PLP.
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Motivated by this hypothesis we decided to investigate the relationship between endogenous PLP and MAL2 in our oligodendrocytic cells using the human HOG cell line.
In hog parietal cells, ClC-5 was found to be localized in both the endosome marker (Rab5 -positive and negative aRab5 -positive, suggesting thandClC-5 was expressed inegativendosomes areasubulovesicles (TV).
For activation of the HOG pathway, yeast cells were treated with 0.4 mM NaCl for 30 minutes as described [14].
BV-2, CCF, HOG, and MO3.13 cell lines were used to study survival of microglia, astrocytes, and oligodendrocytes, respectively.
Analysis of the signaling and transcription dynamics of the HOG pathway in single cells demonstrated that Hog1 activation increases linearly with osmostress, while transcriptional output exhibits a bimodal behavior.
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