Furthermore, the binding of scFv K1 antibody to nmAb-KT was competitive with that of HM-1, i.e., equimolar concentration of HM-1 inhibithe the binding of scFv K1 antibody to the immobilized nmAb-KT antigen.
Normalization was done for expression levels in HM-1 ES cells.
ES cells with E3 or HM-1 as parental line were maintained on mitotically inactivated murine embryonic fibroblasts.
Quantification was normalized for HM-1 mES cells and revealed Utf1 expression levels of around 50% in O9 iPS cells.
The murine bacterial artificial chromosome 189 BAC-189N389N3) strain has been constructed by electroporating HM-1 embryonic stem (ES) cells with the retrofitted BAC-189N3.
Clones UTF-2 and UTF-3 displayed Nanog expression levels that were at least twofold higher than Nanog expression in HM-1 (Fig. 3b).
In addition cDNA from the mES cell line HM-1 was included in the study as well as cDNA from the Oct4-Neo selected iPS cell line O9.
In this respect they behaved like HM-1 IMSS. HM-1 IMSS
Binding of HM-1 was also detected with anti-HM-1 rabbit serum followed by HRP-conjugated anti-rabbit IgG antibody (Promega, USA).
