An additional and fundamental question that remains to be determined before any analysis concerns the choice of the most convenient statistical approach that should be used to identify the best HKG or set of HKG.
Once the more stable HKG or set of HKG in that condition has been evidenced as the best for normalisation, then it will be really possible to begin qRT-PCR experiments.
Taken together, the results of our study suggest that neither a single gold standard HKG nor a set of HKG can be widely used for qRT-PCR normalisation in hypoxia experiments.
In addition, when HEK cells are analysed, whereas GAPDH seems a relevant HKG on the basis of CtCV%, GeNorm and NormFinder considered GAPDH as the worst HKG.
Stein J, Reichert S, Gautsch A, Machulla HKG.
To investigate the stability of various housekeeping genes (HKG) within healthy versus scarred peritoneal mesothelium.
In addition, since the stimulus package focuses heavily on infrastructure and mass transit, she also suggests construction companies like China Railway Construction (HKG: 1186) and steel companies like Angang Steel (HKG 0347).
6. Hong Kong International (HKG).
(Ct (HKG)), where GOI is each gene of interest, and HKG are the housekeeping genes chosen for the "EGF-Control Gene".
(Ct (HKG)), where GOI is each gene of interest, and HKG are the housekeeping genes chosen for the "Sample-Control Gene" worksheet.
Target gene expression was normalized by 18s rRNA (house keeping gene (HKG)) expression.
