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Based on best BLASTX hit criteria that was used previously for barley, these probe sets matched 583 unique rice loci.
They were identified through searches on non-redundant NCBI protein database (September 2008, see Additional file 2 (information A2 for details of search parameters and hit criteria).
These chains can represent both orthologous and paralogous sequences, but are typically filtered with alignment score thresholds or 'best hit' criteria to retain primarily orthologous sequences.
Genes that satisfied BLASTP alignment threshold (35% sequence identity over 80% length of the smallest protein) or bidirectional best hit criteria, were defined as putative orthologs.
For the eQTL analyses, genes were removed if over 5% of the mapped reads were filtered based on the second-best hit criteria above.
Electrophysiological measurements confirmed a hit rate at or above 17%% when the relatively stringent hit criteria of greater than 80%% channel inhibition at 10 μ M was applied.
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To better understand the evolution of this family, we extracted a large set of TGFβ homologues from available animal genome sequences and identified those that had a human inhibin/activin gene as a top hit (this corresponds to the bidirectional BLAST best hit criterion) (Fig. S2A, Table S1).
To ascertain that we identified class 4 HDACs, we first used the 'reciprocal best BLAST hit' criterion.
Nevertheless, they are the closest relatives to the mouse Jmjd6 as defined by the best reciprocal BLAST hit criterion.
HaMStRad maps transcripts to a set of OGs using hidden Markov models and the best reciprocal hit criterion.
After defining the sample size and hit criterion, we initiated a full-scale screen of the JHDL (Chong et al., 2006b, 2006c) using the ARQiv-HTS system.
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