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Hit conditions were defined as those ECMP combinations that supported maximal cell numbers, as well as GFP expression.
We confirmed the ECMP hit conditions by scaling up the 10 top-performing matrix compositions shown in the heatmap of Figure 1B into traditional cell culture formats.
Compared to Matrigel and a sub-optimal matrix (C1 C4 C5 LN), 8 of the 10 ECMP hit conditions significantly increased the percentage of GFP positive cells.
Our observations indicate that this processive polymerase can bypass both stereoisomers of the N BPDE-dA adducts with limited encounters (i.e., under single hit conditions).
Both of the stereoisomers of the N-BPDE-dA adducts were used and reactions carried out under single hit conditions with <30% primer extensions analyzed.
Principal contrasts of interest were (i) for the three hit conditions (neutral, negative, positive) vs. correct rejections (old > new effects), (ii) between a collapsed emotional hit condition (weighted combination of positive and negative hits) and neutral hits (valence-independent effects), and (iii) between positive, negative and neutral hits separately (valence-specific effects).
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The crystallization condition was subsequently optimized by changing the concentrations of precipitant, salts, and buffer around the initial hit condition.
We selected 10 scenes, in which the batter made a grounder hit (HIT condition), and 10 scenes, in which the batter made a ground out (OUT condition).
Contrary, ch-20 and its adjacent channels (ch-13 and 17) were more activated when the supported player lost (OUT condition) than when he won (HIT condition).
For some contrasts, positive and negative hits were collapsed to form a single 'emotional hit' condition.
No GF or SM is shown in comparison to the hit condition CHR + FGF.
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