Sentence examples for hit bead from inspiring English sources

Finally, while highly sophisticated automation exists for analysis by scanning confocal fluorescence microscopy, robotic hit bead selection, and single-bead hit validation prior to resynthesis, there is sparingly little automation for single-bead chemical cleavage and de novo deconvolution of mass spectral data, severely limiting analysis throughput.

Hit beads were washed in water (10 × 2 mL) and re-equilibrated in water overnight.

After a 2 h incubation, hit beads were isolated with a strong magnet.

After 2 h incubation, hit beads were isolated with a strong magnet.

The parafilm was removed from the magnets, and hit beads were washed into a separate vial.

Hit beads were removed using the magnetic pull-down method described above, with careful attention not to lose any of the isolated hits.

A control screen was performed by adding 50 μg mL 1 of pooled Swiss mouse serum to the stripped hit beads in screening buffer.

In this round of screening, the hit beads were saved, stripped of binding proteins by incubating in 0.25% trypsin-EDTA (Life Technologies).

Since hit beads removed in this step represented hits to healthy Swiss mouse IgG, they were discarded, and the nonhit (e.g., nonmagnetized) beads were carried on for further screening.

After the beads were washed with TBS-T (3 × 1.5 mL) and incubated with secondary antibody-coated Dynabeads in screening buffer for 1 h at RT, the magnetic pull-down was repeated once again, and the hit beads were discarded.

Screening a combinatorial solid-phase library entails incubating the library with a labeled target, isolating individual labeled "hit" beads for analysis, and then determining the compound structure on each bead.