Exact(2)
This approach discards reads that do not hit a reference sequence.
For example, clustering sequences de novo produces a gold standard sequence cluster (a robust classification of a taxonomically similar group of sequences), but is very slow, while a reference-based protocol, where sequences are matched in a phylogenetic tree, is very fast but throws out sequences that fail to hit a reference.
Similar(58)
Therefore, there is a high likelihood that insightful results reside in careful analysis of that part of the transcriptome that does not hit a proxy reference genome and for which no known biological function is established.
Transcripts were assigned to unigene clusters based on their best gene hit within a reference species.
"You can't just say, 'I thought it was a kid throwing rocks at my car' and change your story to 'I thought I hit a deer,' " Ms. Griffin said, a reference to reasons that Ms. Nestande gave in the courtroom for leaving the scene.
Wolf and colleagues [ 47], studying carrion crows (Corvus corone), found that 69% of their short-sequence reads hit a zebra finch or chicken reference genome.
The assignment tool GAST was recently reported [40], which uses the best BLAST hits against a reference database of V3 and V6 regions where the taxonomy is known from RDP-classification results.
Scott calls herself a "semi-star" in "semi-hits," a reference to her noted roles in three Broadway shows: Aida, The Little Mermaid and Dirty Rotten Scoundrels.
According to Google Trends, which tracks search terms online, references to Bitcoin hit a record this week.
For reference, a week ago Bitcoin hit an all-time high as it passed $3,000 for the first time.
They treat her big hit as a reference point and as a standard of writerly and box office worthiness.
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