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Our study demonstrates that increased cyclin E expression is associated with distant metastases for whichever histological component of nephroblastoma it is assessed in.
CNS GCTs often present with more than one histological component and are then classified as mixed GCTs [ 7, 10, 11].
Advanced adenoma was defined as any adenoma larger than 9 mm, and/or with a villous histological component higher than 20%, and/or with severe dysplasia.
The microsatellite genotypes of the 27 additional cases were scored according to the level of LOH and the presence of MSI, which were detected in a single tissue piece containing a representative histological component.
All studies included adult patients with good or intermediate risk advanced or metastatic renal cell carcinoma with a clear cell histological component that had not received prior systemic therapy.
In 2005, the Nonalcoholic Steatohepatitis Clinical Research Network (NASH-CRN) Pathology Committee proposed the NAFLD activity score (NAS), which is the sum of each histological component semiquantitatively evaluated as follows: steatosis (0 3), ballooning (0 2), and lobular inflammation (0 3).
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In addition, the primary histology was reevaluated for vascular invasion and per cent composition of histological components of embryonal carcinoma and other histologies.
To the best of our knowledge, the mixed-histology tumor tissues were not macrodissected to separate individual histological components prior to DNA extraction by Oncomatrix.
Specific histological components of the NAFLD disease phenotype were next assessed individually.
Archival blocks were microdissected and individual histological components were subjected to flow cytometry.
We also quantified by morphometry the histological components of the adipose tissue samples from each quadrant in mastectomy specimens.
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