Exactly 0.2 ml aliquots were withdrawn during the incubation at different time intervals up to 6 h and subjected to paper chromatography for determination of the percent of 99mTc-histamine, reduced hydrolyzed technetium and free pertechnetate.
The fact that diphenhydramine, a histamine antagonist, reduced airway symptoms indicates that brevetoxins activate histamine-producing cells, such as mast cells and basophils.
Histamine significantly reduced tonic contractions of uterine strips induced by indolactam V. H1 histamine receptor antagonist abolished relaxation, whereas H2 histamine receptor antagonist had no effect.
Further, depletion of polyamines by culturing BMMCs with α-difluoromethylornithine (DFMO) caused aberrant secretory granule ultrastructure, impaired histamine storage, reduced serotonin levels and increased β-hexosaminidase content.
The results of this study showed that the release of histamine was reduced after OVA challenge in sensitized guinea pigs pretreated with the CTCM.
The amphetamine-induced stereotypies were decreased by administration of the dopamine D2 receptor antagonist haloperidol, and histamine infusion reduced striatal dopamine levels and amphetamine-induced stereotypies.
In addition, the in vivo treatment with histamine significantly reduced the size and the increased exponential doubling time of irradiated triple-negative breast tumors (TNBT) induced in nude mice with MDA-MB-231 cells.
In cellular experiments, the viability of longitudinal layer myocytes from guinea pig ileum was not altered in the presence of XF-OE and the Fluo-4-associated fluorescence intensity in these intestinal myocytes stimulated by histamine was reduced by the essential oil, indicating a [Ca2+]c reduction.
Relatively high concentrations of histamine mimics the effect of clozapine, suggesting the potential role of increased histamine production and reduced HRH4 expression in the tumor growth of CRCs.
Fatigue could be increased by elevated brain serotonin and inflammatory cytokines and reduced histamine binding.
