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As we tromped along, Kevin explained his trapping philosophy.
Redesdale laid out the details of his trapping plan.
His accumulated rage leaks out in his trapping of lizards and his methodical torture of cockroaches.
He called his trapping 1999 Stanley Cup team "kind of slow" and likened it to "an old dog" that waited for opponents' mistakes, then pounced.
Similar(55)
Then she grabbed his hands, trapping them between her stomach and collared shirt.
The wedges fell from the cleft, and the tree closed upon his hands, trapping him.
Oh, and that kid who got his penis trapped in his zip".
The selected variant enzymes were purified by His-trap chromatography as described above.
It was overexpressed in Escherichia coli in a soluble form and purified to electrophoretic homogeneity by His-Trap affinity chromatography.
Lane 1 soluble cell lysate before purification; lane 2 flow-through; lanes 3 12: eluted fractions from His-trap column under reducing conditions.
The crude extract in 50 mM phosphate buffer (pH 7.0) was applied to a His-trap HP affinity chromatography column (GE Healthcare, Piscataway, NJ, USA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com