Sentence examples for his trails from inspiring English sources

The wicking properties of his coat are as important as the comforts offered by comparing his trails and trials to Odysseus or Sir Gawain.

Then, he began waxing philosophic again about the canyon to which he was clearing his trails, saying the spot brought him inner peace -- of a sort.

We've argued over the Lost Dutchman legend while hiking his trails, and have realized that what is real and what is superstition is simply a difference of perspective.

The weather was fair, he wrote, and he was off by early bus to his mountain across the bay, there to climb his trails, staff in hand and knapsack on his back.

The recombinant human His-TRAIL was produced as described previously.

His-TRAIL was quantified by the Bradford method and protein assay reagent (BioRad, Richmond, CA, USA).

Escherichia coli BL21 (DE3) (Invitrogen) was subsequently transformed with the His-TRAIL construct.

Trimeric His-TRAIL was purified by size-exclusion chromatography on a Hiload Superdex 75 column (Amersham Biosciences), to remove impurities.

Twenty-four hours after, cells were stimulated with 50 ng/ml His-TRAIL at 37 °C for the indicated time.

Both His-TRAIL, Bio-NHS-TRAIL and Bio-ULS-TRAIL effectively killed Jurkat cells with IC50 values between 40 ng/ml and 123 ng/ml (Table  1).

The pro-apoptotic activity of His-TRAIL and biotinylated TRAIL derivatives in Jurkat cells was furthermore determined by analyzing caspase 3/7 activity, as shown in Fig.  6b.