Sentence examples for his select from inspiring English sources

Rothschild has won a position as an adviser to Deripaska and one of his select inner circle.

His Select Beads (Sigma) were added and the sample was incubated for 30 min at room temperature.

The poison of its existenceinfects the country's daily life, and we doubt that this is discernible only to Mr. Miller and his select group.

But no organization can function without a consolidation of power in the relationship of a central figure with his select group.

That's the sound of Ryan Detert's invite-only mobile app, Influential, trying to get the attention of one of his select group of users.

Lysates were clarified by centrifugation and incubated with His Select Beads (Sigma) for 30 min with gentle rocking at 4 °C.

(A) Four nanobodies having different sequences were purified by immobilized metal affinity chromatography (IMAC) using a His-Select column.

Soluble sdAbs containing His-tags were purified from the cell lysate by immobilized metal affinity chromatography (IMAC) using a His-Select column Sigma-Aldrich, Saint Louiss, MO, USA).

Rv2745c was affinity purified using His-Select Ni-NTA agarose (Sigma), eluted with imidazole (Sigma) and dialyzed into HRV3C protease buffer (Novagen).

FP was purified from bacterial lysate using an SP-sepharose and a His-Select Nickel Affinity gel (Sigma-Aldrich) and eluted with TUB/100 buffer (60 mM Tris-HCl, 8 M urea, 100 mM imidazole, pH 8.0).

For the Ni2+ pull-down assay, cell lysates obtained as described above, were incubated with 80 µl of His-Select Nickel Affinity Gel (Sigma-Aldrich) during 3 h at room temperature.