C-terminally his-tagged SitC (Fig 5A) was expressed in S. carnosus (pTX30SitC-his), extracted from the cytoplasmic membrane, affinity purified (Fig 6A, lane 1) and labeled with Cy2 (Cy2-SitC-his).
His extracting of the essence of works ranging from "The Mahabharata" to Chekhov's "Cherry Orchard" has sent stern critics into swoons and raised the hackles of the hidebound.
Coley used to inject his extract once or twice a week over a period ranging from a few weeks to several months.
Employing aggressive tactics, threatening to crush his opponents, his office extracted vast civil settlements from defendants eager to avoid criminal indictment.
In April his doctors extracted from his neck one of the bullets he had received from the assassin's gun in August 1918.
His tunes have a melodic directness and simplicity that belie their provocative harmonic turns — which Redd, comping behind his soloists, extracts and emphasizes.
His group extracts and sequences genetic material from fossils, and has produced DNA analyses of both mammoths and Neanderthal man.
He's also changing the way his employer extracts revenue from the stores.
They nosed around his properties like wraiths, interested not in the sundries of his stores or the three grades of gas at his pumps, but in creeping into his head and extracting his secrets.
Winston – before he even gets to room "one-oh-one" – has his fingernails sliced off, his teeth extracted.
