Ty Neil, who declined to give his last name for fear of attracting the attention of TV reporters, said the water washed his truck downstream and trapped him for four hours.
When the flood waters finally receded in the spring, Grant abandoned the canals and marched his army downstream.
He has plenty of disdain for Haiti, along with the usual robust American moralism: "The way Terry saw it, poverty was like a fast-running river sweeping every Haitian and his responsibilities downstream.
He thought of his family, downstream from this spill and still asleep.
A six-histidine tag (small letters) was incorporated in reverse oligo so that Tus will be his-tagged for downstream identification.
For the baculovirus insect cell expression system, a modified pFastBac Dual vector backbone with the melittin secretion signal sequence (Tessier et al. 1991) and an N-terminal HIS-tag encoded downstream of the polyhedrin promoter was used as expression backbone.
Thus, by purifying and then concentrating dA-tailed DNA to reduce the reaction volume and by introducing a prolonged ligation at 4°, the ligation efficiency of the final products was increased from <5 to 63% and provided sufficient material for downstream His-tag bead purification (Table S2).
If Trump does choose a more hawkish monetary policy approach, it will have an ambiguous impact on the dollar, owing to his other proposals' downstream effects.
Additionally, the r1 values of the recombinant viruses were similar to that of the parental virus, indicating that the insertion of FLAG or HIS tag sequences downstream of the RGD motif +9 position do not eradicate the antigenic site of FMDV and do not affect its antigenicity.
Interestingly, 8 out of 13 RBR E3 ligases, including HHARI and HOIP, contain a His two residues downstream of the catalytic Cys, and 6 of these have a negatively charged residue at the position corresponding to Parkin Glu444.
