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And, as recent weeks made clear, Mr Naimi has used his buffer once again.
He has shied away from the necessary one-on-one debate preparation, lately using Alan Keyes as his buffer, yesterday ducking the longstanding invitation of "Meet the Press" to debate his chief opponent only.
Pleased that his buffer relationship is over, Pete goes to claim his second Asian prize and is thwarted by Tyree.
In an effort to establish his buffer relationship as quickly as possible, Pete sends photos of him and his new lady to the digital stratosphere.
When Putin saw even more of his buffer zone eroding with the collapse of the Russian-friendly Yanukovych government in Ukraine, he was tired of the humiliation and acted to salvage what was left by annexing the Russian-speaking area of Crimea back into Russia, which had it taken away in 1954.
The cells were then incubated for 48 h at 37oC before the medium was removed and the cells extracted in high ionic strength (HIS) buffer: 10 mM NaHPO4, pH 7, 1 M NaCl, 1% Triton X-100, 1 mM EDTA.
Similar(48)
But when he had his second child, his financial buffer ran out, and he just could not raise a family on a salary of $40,000.
He was out on his bulldozer Tuesday trying to build his own buffer in case the blaze came his way.
Under the tactful guidance of Monsieur Georges, his invaluable buffer and aide-de-camp, Poiret makes engagements at his office for ten in the morning and keeps them though he may have been seen supping in pretty company at midnight in some distant country village noted for its chef.
His Elution buffer (300 mM imidazole, 50 mM sodium-phosphate pH 8, 300 mM NaCl, 0.01% Tween-20) was added, incubated for 5 min rocking at room temperature, and collected.
This hypothesis, which has been entertained in related forms by multiple researchers, 52, 97, 110 extends Lashley's speculations by positing a discrete neural locus implementing his scannable buffer and by specifying its supraregular nature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com