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Meanwhile, his affinity for business deepened.
And no, he hasn't lost his affinity for the stage.
So his affinity for Victoria Rowell is easy to fathom.
His affinity for underdogs has been no secret.
His affinity for smaller banks traces back to childhood.
Matthew's special friendship with Heaney grew out of his affinity for Ireland and its writers.
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A vector was designed to express CV-N-binding peptides as a fusion with thioredoxin (Trx) containing a penta-His affinity tag.
The reverse primer also contained sequence encoding a C-terminal Hexa-His affinity tag.
The protein was purified with 6x-His affinity Ni II) binding resin (Qiagen, Hilden, Germany).
Briefly, WT PA monomers were mixed with PA F427C (containing a hexa-His affinity tag) monomers in a 40 1 molar ratio.
(3) The 6-histidine (6-His) affinity tag added for Western blot analysis can also be used for purification of selected target proteins using nickel chelating columns.
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