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The highly diversified and duplicated genome hindered sequence assembly and complicated the genome annotation as well as SNP identification.
Furthermore, the nature of highly diversified and duplicated genome of fish species hindered sequence assembly and complicated the genome annotation as well as SNP identification.
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Moreover, it should be noted that prophages often contain homologous sequences inserted at different loci around the genome, hindering sequence assembly efforts.
However, Wang et al. [ 19] used a combination of de novo and alignment methods as the large divergence time between black grouse and domestic chicken (Gallus gallus) used as a reference (~30-40 ~30-40ay hinder sequences fromyaligning properly.
GQ422824 Wheat (Triticum aestivum) is one of the major food crops in the world, providing 20% of the total food calories and protein in human nutrition [ 1] but its large genome size (~16000 Mb) and complexity (~80% repetitive sequences) has hindered genome sequencing studies [ 2].
Apparently, the presence of an inserted domain within the AvrB β-hairpin, combined with an alteration of several active site motif residues hinders sequence recognition.
The large genomes of conifers have greatly hindered their sequencing (but see [ 5]), but they have also prompted large-scale investigations of expressed gene sequences for the inference of putative unigene sets (reviewed by McKay and Dean [ 6]) and initiatives to map these genes (reviewed by Ritland et al. [ 7]).
Those proteins derived from AS share high similarity in sequences and then hinder protein-sequence-based ortholog identification.
These differences may result from copy number variants and chromosomal rearrangements hindering automated sequence assembly of the equine genome [ 21].
Furthermore, very long DNA molecules span complex genomic regions that are rife with repeats that generally hinder accurate sequence assembly without Optical Mapping analysis.
Increasing the sequencing depth was hindered by high sequencing costs, thereby preventing the method to become a broad application.
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CEO of Professional Science Editing for Scientists @ prosciediting.com