In this environment, where anyone can piggyback on the work of others, Mr. Manetas is also concerned that the enforcement of copyrights will hinder expression.
It has previously been shown that selection cassettes located within introns or regulatory regions, such as pgk-neo that we used here, can hinder expression of targeted genes via transcriptional and/or translation interference either by design ([38]; additional examples cited in [39]) or inadvertently [40], [41], [42], which sometimes proves to be serendipitous [42].
These cysteines all contribute to enzymatic activity but can hinder expression of functional enzyme within the reducing environment of the cell interior.
Although identifying the most significant factor hindering expression of ncAA-containing proteins is not always trivial, the most common source of poor expression is suboptimal recognition of the ncAA by the RS.
While it is clear that the UPS is an important cellular defence against toxic polyglutamine protein expression [34], it is less clear how its efficiency can be hindered by expression of mutant polyglutamine proteins.
Since the neo cassette hindered Smn expression as we designed, our alleles can be used to induce Smn expression while still mimicking SMN2 exon 7 alternative splicing.
Rifampicin, being a known inhibitor of gene transcription, is assumed here to have hindered the expression of the toxins by E. coli O157 H7.
RNA interference, for instance, hinders the expression of certain proteins and is, therefore, able to more specifically target the expression of single cytoskeletal elements.
Anti-sense transcription may hinder gene expression from the opposing strand, and therefore, represents a novel mechanism by which NKX3-1 expression may be silenced.
Pakistani liberals now fear a chilling effect that will close minds, hinder reasonable expression and make women timid.Prelude or interlude?
