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The proton effect was found to be of a non-cooperative nature, which was corroborated by a Hill plot that yielded a straight line with a slope (Hill coefficient) of 0.44 (Fig. 6E).
(F) Hill plot of the data.
(B) Hill plot of the data.
The graph revealed a sigmoidal profile and allosteric behaviour (Fig. 1E) and affinity for the substrate in the μM range with positive cooperativity, as indicated by the Hill plot (Fig. 1F).
The Hill plot obtained by the Enzyme Kinetic Module 1.1 (SPSS Science Software) was used to determine the apparent S0.5 and nH values.
Initial velocity studies at different AdoMet concentration showed cooperative (sigmoidal) behavior and Hill plot analysis showed that two molecules of AdoMet binds to HP0593 MTase.
Similar(43)
(1) ν / [ E ] = k cat,app [ NRH ] / K m,app + [ NRH ] Linearized Hill plots were constructed to determine the Hill coefficient (h) according to Eq. (2).
Hill plots were used to calculate the Hill coefficient (nH), the maximal velocity (Vmax), and the kinetic constants that correspond to the activator or substrate concentrations giving 50% of the maximal activation (A0.5), or velocity (S0.5).
Both BChE species displayed biphasic hill plots using butyrylthiocholine as substrate.
Similar effects of chain heterogeneity may give rise to disproportionate reductions in the slopes of Nernst and Hill plots for hemoglobins (Hbs).
Comparison of Nernst plots for redox data with Hill plots for O2 binding data shows that the redox process is more affected by the structural differences in the distal heme pockets of the Mbs studied than is O2 binding.
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