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Three deaths (probable and confirmed cases combined) occurred in two of the four highly significant clusters of probable and confirmed cases of LACV infections in West Virginia and Tennessee.
There was a strong decline in mortality, and two highly significant clusters of increased mortality were found for the first 5 years, 1998 2002, in the south-east and in the north of the study area.
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We would anticipate the observed, highly significant clustering of SNPs that display nominally positive results if many of these reproducibly positive SNPs lay near and were in linkage disequilibrium with functional allelic variants that distinguished substance dependent subjects from control subjects, but not if they represented chance observations.
Enrichments of DNA sequences were observed across larger domains of genes, and assessment of topologically associated domains showed highly significant clustering of escapes and subjects within such domains.
Given the relative proportion of HV regions along the sequences, this represents a highly significant clustering of sites within HV regions and CVR (p = 0.0009; hypergeometric distribution).
Phylogenetic analysis of the comparatively long groEL gene sequence showed highly significant clustering of the samples into 3 groups supported by high bootstrap values, suggesting the existence of at least 3 types of Candidatus N. mikurensis sequence variants.
It is noteworthy that the probe sets located in 2p were substantially enriched in the highly significant clusters 9 of A549 (p = 0.00032) and 5 of Beas-2B (p = 0.000114) (data not shown).
Second, by means of the negative binomial distribution a p-value is assigned to each CpG cluster, which allows the prediction of highly significant clusters such as CpG islands.
Among the identified positional clusters, there are ten highly significant clusters containing eight or more genes, listed in Table 3.
Bootstrap values of >700 (70%) indicate the highly significant clustering, whereas values <500 (50%) indicate that the clustering is not statistically significant.
Highly significant clustering was also apparent according to diet which could be analysed as a variable independently of host phylogeny.
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