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Seven samples were run as experimental replicates to estimate assay reproducibility for which an average Pearson's R of 0.965 indicated highly reproducible data (Supplementary Figure 1).
All measurements were made at least three times for each test, and highly reproducible data were obtained within the coefficients of variation of ±5% in all cases.
Highly reproducible data were obtained as the coefficients of variation of threshold cycle values in inter-runs were less than 2.2%.
ELISPOT assays provide robust, highly reproducible data and can be retested for the acquisition of additional information in follow-up assays".
A CV of 0.15 or less generally indicates highly reproducible data, and thus, we were satisfied that replication is an effective strategy to reduce variability for our analysis of mass spectra derived from serum.
Both methods generate unambiguous and highly reproducible data.
Similar(31)
We conclude that the knowledge acquired from early work on the technical issues related the protocols and data processing methods applied to gene expression microarrays have greatly facilitated the rapid implementation of highly-reproducible data using miRNA arrays.
Although NGS platform has been shown to generate highly reproducible, accurate data with high correlation to other platforms for high quality RNA samples [ 21– 24], comparative data on media with low quality and quantity RNA are lacking.
Our highly reproducible hybridization data confirm also the sufficient thermostability of the immobilized mod-Ph.
The designed RCM is demonstrated to provide highly reproducible experimental data at compressed gas pressures up to 100 bar.
As the biological replicates are highly reproducible, the data should be consistent and comparable to the other conditions.
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