We found that highly replication-restricted modified vaccinia virus Ankara (MVA) that was engineered to produce excess amounts of dsRNA either from EGFP gene inserts or from the endogenous E3L gene very early in infection showed enhanced induction of IFN-β in murine and human cells, despite a functional E3L gene.
SHIV-2873Nip is a highly replication-competent, mucosally transmissible, pathogenic R5-tropic virus that will be useful to study viral pathogenesis and to assess the efficacy of immunogens targeting HIV-C Env.
The results indicate that while point mutations are highly replication-dependent, genome rearrangements are as easily induced in non-dividing cells as in mitotically active ones.
The results demonstrate that the Sulfolobus chromosome is organised in a highly replication-biased manner, such that levels of gene expression, as well as genome sequence-derived parameters, are correlated with distance to nearest replication origin.
Hepatitis C virus (HCV) replicates in membrane associated, highly ordered replication complexes (RCs).
However, we also showed that there are regions within the genomic landscape of budding yeast with highly specific replication rates, which cannot be explained by global properties of the replication machinery.
To this end, we introduce a highly distributed replication technique that determines optimal data locations to improve access performance by minimizing replication overhead (access and update).
One of the most highly connected replication fork proteins is Ctf4, which appears to function as a scaffold during DNA replication and repair, acting as a hub of protein−protein interactions (Im et al. 2009; Mimura et al. 2010; Zhu et al. 2007).
Mcm family members are highly conserved replication initiation factors assembling with Cdt1 and Cdc6 proteins into pre-replicative complexes at origin recognition complexes during G1 phase (Maiorano et al, 2006), thereby licensing chromatin for replication in the subsequent S phase.
Despite the highly attenuated replication in vivo, the mutant completely protected the animals against the wild type virus challenge.
