They suggest a strategy of "extensive sequence-based sampling of germplasm collections using highly parallel technology" combined with the elegant progenitor-based comparison method they describe, largely driven by T. occidentale, since T. pallescens seems to be less similar to the P' sub-genome The results above suggest a complementary strategy.
This highly-parallel technology has enabled thousands of genes or tens of thousands of single nucleotide polymorphisms (SNPs) to be recovered in a single experiment [ 1].
Highly parallel sequencing technologies have become important tools in the analysis of sequence polymorphisms on a genomic scale.
Custom-designed microarrays, termed resequencing arrays (RAs), are a highly parallel emerging technology with a rapidly diminishing cost basis that can be used to perform DNA sequencing by hybridization [18] [21].
Within the CRoPS system, tagged complexity-reduced fragment libraries generated by the AFLP process [ 14] was sequenced by 454 highly parallel sequencing technology, and the sequences were aligned and mined for polymorphisms.
Molecular marker technologies, however, are currently undergoing a transition from largely serial technologies based on separating DNA fragments according to their size (SSR, AFLP), to highly parallel, hybridization-based technologies that can simultaneously assay hundreds to tens of thousands of markers (e.g., Single Nucleotide Polymorphisms or SNPs) [ 12].
These datasets are constructed by correlating expression levels of genes, usually measured using a highly parallel expression profiling technology, with the presence of different SNPs.
Highly parallel, 'second generation' sequencing technologies have rapidly expanded the number of bacterial whole genome sequences available for study, permitting the emergence of the discipline of population genomics.
Subsequently, the circularized target sequences are amplified in multiplex and analyzed by using a highly parallel sequencing-by-synthesis technology.
In their exponential growth, they require new integrative technologies for highly parallel analysis of genes and proteins of entire organisms.
