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Φ-value analysis of the unfolding data revealed a highly native transition state.
Even under highly native conditions, structured proteins have access to a manifold of near-native conformations.
These data may appear at odds with the finding of Fitzpatrick and Shaffer [ 38] that viability selection in the wild favors the most genetically intermediate individuals, regardless of whether the pre-selection population is highly native or nonnative.
Thus, what Fitzpatrick and Shaffer [ 38] demonstrated is that selection on a given larval cohort seems to favor admixed individuals at the expense of highly native and highly nonnative contemporary hybrids.
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TS2 is highly native-like (αTS2 = 0.93) and represents a late entropic barrier to formation of the native state.
Although the non-native amyloid precursor of β2-microglobulin is highly native-like, its edge strands contain perturbations that normally protect β-sandwich proteins from self-association [43] [46].
We also need to be careful about how we define non-native contacts, as they could involve a range of different scenarios, from the slight re-organization of sidechain packing in a structure that is otherwise highly native-like, to the formation of interactions far from those that define the native structure.
The Millipore anti-GFP antibody is made against highly purified native GFP from Aequorea victoria and reacts with both native and recombinant GFP sources.
As part of a broad network of chaperones, they appear to be able to disassemble stable complexes; to unfold highly stable native protein domains; and to help to resolubilize and refold non-native proteins trapped in a high molecular weight aggregate [32].
Later, as he examined what appeared to be highly unusual Native American artifacts found at the site, his doubt deepened.
Education cutbacks have meant that young Uighurs are not learning functional Chinese, as highly paid native Chinese speakers have been replaced by cheaper local teachers.
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