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Stable isotope-labelling enrichment has been analysed by GC MS results showing that the biomass is highly labelled.
Acetyl-CoA should be highly labelled because it is generated from the rapidly labelled PEP pool via the final steps of the glycolytic pathway.
Gating settings were set to collect highly labelled cells.
Experiments using radiolabelled arachidonic acid demonstrated that the nuclear membrane is highly labelled after cellular administration [ 39].
It should be noted, however, that these proteins, GrpE and GroES, were highly labelled with phosphate in the extracts obtained from cells grown at the normal temperature.
Label from glucose is rapidly incorporated into highly labelled diffusible metabolites, mainly lactate that quickly carry substantial amounts of label out of activated tissue to blood.
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SILAC pulse-chase labeling experiments show that nascent MRPs recruited to the nucleoid fraction were highly labeled after the pulse in a transcription-dependent manner and decreased in labeling intensity during the chase.
These clusters were highly labeled with anti Ki67 antibody indicating a high proliferation index.
This flow cytometry data would correspond to the highly labeled small size cells seen by fluorescence microscopy (Figs. 7A F).
Interestingly, the central canal area (lamina X) was highly labeled in lumbar spinal cord segments that receive primary afferent input from the affected femoral bone (Fig. 4F).
PrPd brain mapping analyses revealed several characteristic features: i) PrPd accumulated almost exclusively as plaques (Figure 4A1); ii) the brainstem, cerebellum and cochlear nucleus (Figure 4A3) did not show any PrPd accumulation whereas the thalamus, midbrain and cortex presented PrPd plaques (Figure 4A) and iii) the rostral region of the cortex was markedly more highly labeled.
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