Reverse primers are more highly exhausted by one-step than two-step qPCR on account of their being incorporated into the amplicons during first-strand synthesis (16).
The battle of the biennials is proving to be highly entertaining, but exhausting.
During the four days that I spent on Bear Island, it often occurred to me that the highly stimulating and occasionally exhausting "Bear Island atmosphere" that Fuller had talked about the first night was more or less directly the product of his own presence there.
CD244, also known as 2B4, was firstly described as an inhibitory receptor highly expressed on exhausted CD8+ T cells in line with PD-1 in chronic LCMV infection and chronic HBV infection in mice.
This gene encodes a ligand that, in combination with its receptor PD-1, delivers inhibitory signals that modulate the activation state of monocytes and CD4+ T cells [ 52] and is highly expressed on exhausted T cells during chronic viral infections [ 53].
It is a well-known fact that a turbocharger turbine in an internal combustion engine operates in a highly pulsating exhaust flow.
Rural kitchens were equipped with low-efficiency chimneys compared with the highly efficient exhaust fans in urban kitchens.
Meldrum-Hanna: "It was an exhausting shoot and a highly stressful pressure-cooker environment".
PD-1 is also highly-expressed on exhausted CD8+ T cells (Barber et al., 2006; Youngblood et al., 2013).
He has been bombastic and demagogic, exhausting and occasionally apologetic, and almost always highly effective.
The available sources, while exhausting and not friendly to the environment, are highly used.
