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Its function is consistent with our finding that it is also highly deleted in AA CRC patients.
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The manipulation of CNE5 (Figure 6) revealed that the deleted highly conserved element encompassing predicted contiguous TFBSs for developmental regulators PBX1, PAX2 and MEIS1 is necessary but not sufficient for the activating potential of this site.
The mAgo5 open reading frame (ORF) is fragmented with some regions being either highly divergent or deleted relative to other Argonaute proteins.
We identified 181 amplified and 536 highly variable or deleted genes or fragments, 74 (40.9%) and 30 (5.6%) of which, respectively, were reported previously [ 28, 29, 33].
In the linear decreasing phase of population׳s number, some highly similar individuals are deleted.
Highly variable regions were deleted, and alignments were modified by hand using Seaview (Galtier et al. 1996; http://pbil.univ-lyon1.fr/software/seaview.html, last accessed April 10 , 2013.
The enrichment for D. melanogaster-biased features suggests that genes in these categories are either highly diverged or are deleted in D. yakuba.
The full sequence alignments of all Msx orthologs and paralogs (Additional File 1 page 1 8) revealed that the coding region that contained the MH1C domain in Msx1 and Msxe orthologs appeared to be deleted or highly diverged in the Msx2/a/d and Msx 3/b/c paralog groups (Additional File 1 page 4).
Moreover, the extent to which p16 was deleted in smears highly correlated with the extent of p16 deletion in tissues.
For each alignment, we obtained a dataset that was as complete as possible after deleting highly polymorphic indel regions.
Another strategy that can help is to delete highly variable sequence positions to reduce the number of non-homologous similarities.
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