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Sequence analysis demonstrated α-Syn peptides (yellow highlighted sequences, Figure 1C) in the VMB from both PBS- and MPTP-treated mice but exclusively in the CLN of MPTP-intoxicated mice (Table 1).
Blue highlighted sequences indicate Shaw-Kamens sequences, also known as AU-rich elements (AREs [Shaw and Kamen, 1986]).
The protein sequence coverage by the HRMS (yellow highlighted sequences in Figure 4) on the five samples ranged from 58.66% (human CIα1) to 85.81% (Pichia CIα1-OH).
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Yellow highlighted sequence denotes H4k20me1 histone modification (NHEKs).
Yellow highlighted sequence denotes a DNase I hypersensitive site and Faire-seq peak (NHEKs).
Yellow highlighted sequence denotes a DNase I hypersensitive site (Th17 cells).
The names of all RT-PCR primers are listed above the highlighted sequence, and the arrows indicate whether they are forward or reverse primers.
Both the NCBI and Ensembl 3'UTRs were cloned using the red highlighted primer sequences in Figure 3A into a luciferase reporter vector and named luc-CDH11 3'UTR NCBI and luc-CDH11 3'UTR Ensembl, respectively.
The colored underlines correspond to the colored highlighted conserved sequences in panel A).
Figure 10 shows two breakpoint sequences (highlighted in red) that straddle a high A + T sequence (highlighted in blue).
Cellulases affiliated with GH9 are highlighted in green, GH12-affiliated sequences are highlighted in blue, GH5-affiliated sequences are highlighted in red and GH1-affiliated sequences are highlighted in yellow.
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CEO of Professional Science Editing for Scientists @ prosciediting.com