To avoid matching paralogous genes from a source organism, we include only the highest-scoring gene from a source organism.
The highest-scoring transcription factor motif identified by this study was nuclear respiratory factor 2 (NRF-2, also known as GABP) (Rosmarin et al., 2004) from the neocortex dataset, regardless of number of genes analyzed (Fig. 1D– F; for the other datasets, Fig. S1 (Supporting information); full results for all datasets are in File S3, Supporting information).
Eleven proteins were identified as potential targets of BIA 10-2474, and the two highest-scoring proteins, Factor VII and thrombin, both essential blood-clotting factors, were predicted to be inhibited by BIA 10-2474 and suggest a plausible mechanism of toxicity.
For the highest-scoring transcription factors we cross-validated the expression pattern by looking for the number of corresponding tags in the LongSAGE libraries.
Screening the human death-associated protein 3 (hDAP3) gene promoter with the 125 TFBS motifs, these nine transcriptional factors presented the highest-scoring sites (Table 2).
Therefore, we defined a "confidence score" as the difference between the individual classification scores for the two highest-scoring tissues.
The motifs showing the highest-scoring TFBS detached from the background concerned the nine transcriptional factors (or combinations of transcriptional factors) extracted earlier with the analysis of gene promoters.
He also had his highest-scoring game ever that season, scoring 20 points against Georgetown College.
On the BVMT, control subjects on insulin were the highest-scoring subgroup, while DM subjects on placebo scored the lowest.
The highest-scoring predicted target using the miRanda algorithm [ 31] was the gene encoding the Ets transcription factor Friend leukemia integration 1 (Fli1).
His highest-scoring play was the time he dropped OBLIQUED across two triple-word scores for 230 points.
