Sentence examples for highest transformation frequency from inspiring English sources

The hypervirulent A. tumefaciens strain AGL1 carrying the binary vector pDM805 containing the bar gene under the control of the Ubi1 promoter and the uidA gene driven by Act1 promoter was used for transforming four different explant types of which somatic embryos gave the highest transformation frequency [ 47].

As shown in Figure 7A, the highest transformation frequency (91.54%) was achieved with a 2-day co-cultivation.

The highest transformation frequency (74.64%) was obtained when the stem sections with one node were used as explants.

The highest transformation frequency was found when the co-cultivation was carried out at 22°C in this study.

The highest transformation frequency (91.67%) was observed after a 2 days pre-culture and it was remarkably different from the other pre-culture duration (P < 0.05).

The highest transformation frequency (89.64%) was obtained when A. rhizogenes cultures at the late-log stage were used, corresponding to OD600 = 0.6.

The three DNA positions that exhibited the highest transformation frequencies (TCP3, TCP7 and TCP11) contained two copies each of the consensus sequence (Table 1).

In spite of the increasing range of transformation frequencies with increasing genomic divergence, the DNA positions that yielded the highest transformation frequencies with GMI1000 (TCP3, TCP6, and TCP7) systematically yielded the highest frequencies for each recipient (Figure 5).

Similarly, the highest transformation frequencies were observed when recombination was mediated by DNA positions (e.g., gTCP3 and gTCP7), which also yielded the highest frequency when they were located on a plasmid.

Therefore, the reduced differences found between the lowest and the highest transformation frequencies with genomic borne fragments could be explained by the involvement of Chi-like sequences at some unknown distance upstream.

Therefore, we attempted to elucidate the mechanism of high transformation frequency in CAG18439 as follows (Table 7 and Figs. 3 6).